The quick detection of the bacterial antigens in a person’s bodily fluids is done by immunological tests like the latex agglutination test. Although the detection of the antigenuria by the latex agglutination has the theoretical appeal, the troublesome reactions like the nonspecific agglutination of the latex particles have a limited utility of technique. It was thus recently discovered that the false positive reactivity is common in the latex agglutination test. This was used to detect the influenza type ‘b’ antigen present in the urine at the time the urine was prepared by filtering through some of the membrane filters. A rapid technique is then disclosed for an identification of the enteric bacteria that elaborate cholera-related heat-labile enterotoxin antigens. These have an ability to cause diarrheal disease in either man or in the animals. This invention includes the sensitized carrier particle for the use as the reagent in the agglutination test for the heat-labile enterotoxins and also the use of the said reagent in the immunological determinations.
Acute pharyngitis is found to be one of the most common among the diseases in a pediatric practice, and also, the most common among bacterial etiology is the group A beta-hemolytic streptococcus or GABHS. The study was conducted to check the accuracy of the GABHS rapid antigen detection test while comparing with the oropharynx swab culture. The rapid test that was studied showed a good correlation with the culture and is, thus of great use in the clinical practice for the detection of GABHS. Acute pharyngitis or AP is one of the most common diseases in pediatric practice. Thus the early diagnosis of this infection, is followed by the appropriate antimicrobial treatment, and is extremely relevant to prevention of the rheumatic fever or the RF and the rest of the suppurative complications. The swabs were taken simultaneously both from the tonsils and the posterior pharyngeal region. The latex particle agglutination was performed on one of the two swabs. The second swab was then used to seed an agar and the 5%lamb’s blood plate within the 20 minutes, which was then incubated for upto18 to 24 hours in themicroaerophilic conditions at 37 ºC. The plate was then interpreted based on the colony morphology and the hemolysis pattern by the microbiologist who did not know the result of that latex test. Suspect colonies were then confirmed as the beta-haemolytic streptococcus by means of the latex agglutination test.